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Anti-CD101抗體;CD101抗體簡單介紹:
產品編號:BYK-10727R
英文名稱:Anti-CD101
中文名稱:CD101抗體
別名:IGSF2; Immunoglobulin superfamily member 2; Leukocyte surface protein; V7; V7 LSB; IGSF2_HUMAN; IgSF2; Cell surface glycoprotein V7; Glu-Trp-Ile EWI motif-containing protein 101; EWI-101; CD101.
背景介紹:CD101 is a disulfide-linked homodimeric type 1 glycoprotein. The peptide is comprised of 7 extracellular V-type IgSF domains. CD101 is highly expressed on monocytes, granulocytes, mucosal T cells, and on activated peripheral blood T cells. Expression is weak on resting T and B and NK cells, absent from plaets and weak or absent from most hematopoietic cell lines. CD101 is thought to play a co-stimulatory role in TCR/CD3-mediated T cell activation. Monoclonal antibodies against CD101 inhibit allogeneic T cell responses. Studies suggest that CD101 plays a major role in the activation of T cells by skin dendritic cells.
產品規格:50ul;100ul;200ul;1ml,*
抗體來源 Rabbit or Mouse
克隆類型 Polyclonal or Monoclonal
交叉反應、產品應用 (具體交叉反應與產品應用請或添加客服直接索取)
性 狀 Lyophilized or Liquid
濃 度 1mg/ml
Anti-CD101抗體;CD101抗體產品信息:
SABC法
- 脫蠟、水化
- PBS洗2次各5分鐘
- 用蒸餾水或PBS配制新鮮的3% H2O2 ,室溫封閉5-10分鐘,用蒸餾水洗3次
- 抗原修復
- PBS洗5分鐘
- 滴加正常山羊血清封閉液,室溫20分鐘,甩去多余液體
- 滴加Ι抗50ul,室溫靜置1小時或4℃過夜或37℃1小時
- PBS洗3次各2分鐘
- 滴加*化Ⅱ抗,20℃-37℃ 20分鐘
- PBS洗3次各2分鐘
- 滴加試劑SABC 20℃-30℃ 20分鐘
- PBS洗4次各5分鐘
- DAB顯色:試劑盒或自配顯色劑顯色
- 脫水、透明、封片、鏡檢
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