Anti-TNXB抗體-腱糖蛋白X抗體簡單介紹：

產品編號：BYK-18163R

英文名稱：Anti-TNXB
中文名稱：腱糖蛋白X抗體

別名：Ehlers Danlos like syndrome; Hexabrachion like protein; Hexabrachion-like protein; HXBL; NXB2; Tenascin X precursor; Tenascin XB; Tenascin XB1; Tenascin XB2; Tenascin-X; TENX; TENX_HUMAN; TN X; TN-X; TNX; TNXB; TNXB1; TNXB2; TNXBS; XB; XBS.  

背景介紹：This gene encodes a member of the tenascin family of extracellular matrix glycoproteins. The tenascins have anti-adhesive effects, as opposed to fibronectin which is adhesive. This protein is thought to function in matrix maturation during wound healing, and its deficiency has been associated with the connective tissue disorder Ehlers-Danlos syndrome. This gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. It is one of four genes in this cluster which have been duplicated. The duplicated copy of this gene is incomplete and is a pseudogene which is transcribed but does not encode a protein. The structure of this gene is unusual in that it overlaps the CREBL1 and CYP21A2 genes at its 5' and 3' ends, respectively. Multiple transcript variants encoding different isoforms have been found for this gene.

產品規格：50ul；100ul；200ul；1ml，*

抗體來源 Rabbit or Mouse

克隆類型 Polyclonal or Monoclonal

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性    狀 Lyophilized or Liquid
濃    度 1mg/ml

Anti-TNXB抗體-腱糖蛋白X抗體產品信息：

石蠟切片免疫組化染色步驟

1、三步法（以SP試劑盒為例）

- 石蠟切片脫蠟至水

- 蒸餾水沖洗，PBS浸泡5分鐘，如果采用抗原修復，可在此步后進行

- 3% H2O2 室溫孵育5~10分鐘，以消除內源性過氧化物酶的活性，PBS沖洗，2分鐘×3次

- 5~10%正常山羊血清封閉，室溫孵育10分鐘。傾去血清，勿洗，滴加適當比例稀釋的一抗或一抗工作液，37℃孵育1~2小時或4℃過夜

- PBS沖洗，2分鐘×3次

- 滴加適當比例稀釋的*標記二抗（1%BSA-PBS稀釋），37℃孵育10~30分鐘；或滴加第二代*標記二抗工作液，37℃或室溫孵育10~20分鐘

- PBS沖洗，2分鐘×3次

- 滴加適當比例稀釋的辣根酶標記鏈霉卵白素（PBS稀釋），37℃孵育10~30分鐘；或第二代辣根酶標記鏈霉卵白素工作液，37℃或室溫孵育10~20分鐘

- PBS沖洗，2分鐘×3次

- 顯色劑顯色（DAB或AEC）

- 自來水充分沖洗，復染，脫水透明、封片

- 如果必要，可選用avdin封閉內源性*

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