蘇州阿爾法生物實驗器材有限公司
蘇州阿爾法生物實驗器材有限公司
人視網膜色素上皮細胞ARPE19該細胞系系源自于一名19歲車禍罹難的健康男性的視網膜組織，由AmyAotaki-Keen建系于1986年。該細胞系表達視網膜色素細胞的分子標記如胞內結合蛋白和PRE-65。
人視網膜色素上皮細胞 ARPE19
人視網膜色素上皮細胞 ARPE19 該細胞系系源自于一名19歲車禍罹難的健康男性的視網膜組織，由AmyAotaki-Keen建系于1986年。該細胞系表達視網膜色素細胞的分子標記如胞內結合蛋白和PRE-65。
細胞名稱：人視網膜色素上皮細胞
細胞簡稱：ARPE-19
細胞別稱：ARPE19; Adult Retinal Pigment Epithelial cell line-19; NTC-200; NTC200
產品貨號：TCH-C127
規格：1×10?cells T25細胞培養瓶
種屬來源：人
組織來源：視網膜
細胞形態：上皮細胞樣
生長特性：貼壁生長
培養體系：DF-12+10%FBS+1%P/S
配套培養基貨號：TCH-G127
傳代比例：1:3-1:6，每2-3天換液一次
傳代周期：48-72 h
培養條件：氣相：95%空氣+5%CO2，溫度：37℃
凍存條件：60%基礎培養基+30%FBS+10%DMSO，液氮儲存
質量檢測：細菌、真菌、支原體檢測均為陰性
實驗室儀器及實驗耗材：二氧化碳培養箱，細胞培養皿，細胞培養瓶，細胞培養搖瓶
人視網膜色素上皮細胞 ARPE19 使用說明書
參考文獻：
"Hazim RA, et al. Rapid differentiation of the human RPE cell line, ARPE-19, induced by nicotinamide. Exp Eye Res 179: 18-24, 2018. PubMed:
Dunn KC, et al. ARPE-19, A human retinal pigment epithelial cell line with differentiated properties. Exp. Eye Res. 62: 155-169, 1996. PubMed: 8698076
Maidji E, et al. Accessory human cytomegalovirus glycoprotein US9 in the unique short component of the viral genome promotes cell-to-cell transmission of virus in polarized epithelial cells. J. Virol. 70: 8402-8410, 1996. PubMed: 8970961
Holtkamp GM, et al. Polarized secretion of IL-6 and IL-8 by human retinal pigment epithelial cells. Clin. Exp. Immunol. 112: 34-43, 1998. PubMed: 9566787
Finnemann SC, et al. Phagocytosis of rod outer segments by retinal pigment epithelial cells requires alpha(v)beta5 integrin for binding but not for internalization. Proc. Natl. Acad. Sci. USA 94: 12932-12937, 1997. PubMed: 9371778
Handa JT, et al. The advanced glycation endproduct pentosidine induces the expression of PDGF-B in human retinal pigment epithelial cells. Exp. Eye Res. 66: 411-419, 1998. PubMed: 9593635
Dunn KC, et al. Use of the ARPE-19 cell line as a model of RPE polarity: basolateral secretion of FGF5.. Invest. Ophthalmol. Vis. Sci. 39: 2744-2749, 1998. PubMed: 9856785
Tugizov S, et al. An acidic cluster in the cytosolic domain of human cytomegalovirus glycoprotein B is a signal for endocytosis from the plasma membrane. J. Virol. 73: 8677-8688, 1999. PubMed:
Orten DJ, et al. Analysis of DNA elements that modulate myosin VIIA expression in humans. Hum. Mutat. 14: 354, 1999. PubMed:
Rajan PD, et al. Expression of the extraneuronal monoamine transporter in RPE and neural retina. Curr. Eye Res. 20: 195-204, 2000. PubMed:
Janssen JJ, et al. Retinoic acid delays transcription of human retinal pigment neuroepithelium marker genes in ARPE-19 cells. Neuroreport 11: 1571-1579, 2000. PubMed:
Udono T, et al. Adrenomedullin in cultured human retinal pigment epithelial cells. Invest. Ophthalmol. Vis. Sci. 41: 1962-1970, 2000. PubMed:
Sparrow JR, et al. The lipofuscin fluorophore A2E mediates blue light-induced damage to retinal pigmented epithelial cells. Invest. Ophthalmol. Vis. Sci. 41: 1981-1989, 2000. PubMed: "
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