蘇州阿爾法生物實驗器材有限公司
蘇州阿爾法生物實驗器材有限公司
Jurkat細胞是Jurkat-FHCRC細胞株(Jurkat細胞株的衍生)的一個克隆。生長方式為懸浮生長，生長速度較快，使用1640培養液培養。顯微鏡下觀察，正常細胞狀態為圓潤透亮，似魚卵。
Jurkat細胞
細胞名稱： 人T淋巴細胞白血*細胞（STR鑒定正確）
細胞簡稱：Jurkat細胞
細胞別稱：JurkatE6-1; Jurkat E6-1; Jurkat, Clone E6-1; Jurkat Clone E6-1; Jurkat (clone E6-1); JURKAT E-6.1; JURKAT E-61; Jurkat-E6; Jurkat E6; J.E6-1; E6-1
貨號：TCH-C225
規格： 1×10?cells/T25 培養瓶
價格：1500元 (干冰運輸需另加200元干冰費)
種屬來源：人
組織來源：外周血
特征：急性T淋巴細胞白血*
細胞形態：淋巴母細胞樣
生長特性：懸浮生長
培養體系：RPMI-1640+10%FBS+1%Glutamax+1% Sodium Pyruvate+1%P/S
Jurkat細胞 配套培養基貨號：TCH-G225
傳代比例：1:3-1:6，每2-3天換液一次
傳代周期：24-48 h
培養條件：氣相：95%空氣+5%CO2，溫度：37℃
凍存條件：60%基礎培養基+30%FBS+10%DMSO，液氮儲存
質量檢測：細菌、真菌、支原體檢測均為陰性
該細胞是Jurkat-FHCRC細胞株(Jurkat細胞株的衍生)的一個克隆。生長方式為懸浮生長，生長速度較快，使用1640培養液培養。顯微鏡下觀察，正常細胞狀態為圓潤透亮，似魚卵。
Jurkat細胞株來源于一個14歲男孩的外周血；經弗波酯和外源凝集素或抗T3單克隆抗體中的一種誘導后可產生大量IL-2(IL-2的產生需兩種類型的誘導劑)；表達T細胞受體、CD3。
蘇州阿爾法生物實驗器材有限公司除了提供細胞系購買，細胞株購買，pc12細胞株，肺癌細胞株，Jurkat細胞，血管內皮細胞株，肝癌細胞株，腫瘤細胞株，胃癌細胞株，hela細胞株，胰島細胞株，s180細胞株，hela細胞株，動物細胞，干細胞外，還提供MEM培養基,DMEM,F12培養基,RPMI培養基等基礎培養基，干細胞培養基,培養基添加劑,間充質干細胞培養基 ,培養基,低血清培養基,胎牛血清,雞血清等細胞培養試劑。
參考文獻：
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Chan YJ, et al. Synergistic interactions between overlapping binding sites for the serum response factor and ELK-1 proteins mediate both basal enhancement and phorbol ester responsiveness of primate cytomegalovirus. J. Virol. 70: 8590-8605, 1996. PubMed: 8970984
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