蘇州阿爾法生物實驗器材有限公司
蘇州阿爾法生物實驗器材有限公司
細胞名稱：人結腸癌細胞（STR鑒定正確）細胞別稱：HT 29; HT29；ht-29細胞細胞貨號：TCH-C213規格：1×10?cells | T25 培養瓶配套培養基貨號：TCH-G213
ht-29細胞
細胞名稱： 人結腸癌細胞（STR鑒定正確）
細胞別稱：HT 29; HT29； ht-29細胞
貨號：TCH-C213
規格：1×10?cells | T25 培養瓶
價格：1500元 (干冰運輸需另加200元干冰費)
種屬來源：人
組織來源：結直腸
特 征：結直腸癌
細胞形態：上皮細胞樣
生長特性：貼壁生長
培養體系：McCoy's 5A+10%FBS+1%P/S
配套培養基貨號：TCH-G213
傳代比例：1:3-1:6，每2-3天換液一次
傳代周期：48-72 h
培養條件：氣相：95%空氣+5%CO2，溫度：37℃
凍存條件：60%基礎培養基+30%FBS+10%DMSO，液氮儲存
質量檢測：細菌、真菌、支原體檢測均為陰性
ht-29細胞是1964年由FoghJ用移植培養方法和含15%FBS的F12培養液從原發性腫瘤分離的。近來，已建株的培養細胞用含血清的McCoy's5a培養基培養。該細胞系在裸鼠中成瘤，也能在類固醇處理的地鼠中成瘤。該細胞可合成IgA、CEA、TGFβ結合蛋白和黏液素；表達受體，但沒有檢測到血漿酶原活性；不表達CD4，但細胞表面表達半乳糖神經酰胺（HIV的可能替代受體）。該細胞系癌基因c-myc、K-ras、H-ras、N-ras、Myb、sis、fos陽性；p53基因過表達，并且在273位密碼子處發發生G。
蘇州阿爾法生物除了提供細胞系購買，細胞株購買，pc12細胞株，肺癌細胞株，ht-29細胞，血管內皮細胞株，肝癌細胞株，腫瘤細胞株，胃癌細胞株，hela細胞株，胰島細胞株，s180細胞株，hela細胞株，動物細胞，干細胞外，還提供MEM培養基,DMEM,F12培養基,RPMI培養基等基礎培養基，干細胞培養基,培養基添加劑,間充質干細胞培養基 ,培養基,低血清培養基,胎牛血清,雞血清等細胞培養試劑。
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