| 主要功能 | |||||
| 1. 專業分析浮游植物細胞，同時具備傳統流式細胞儀經典功能 |  | ||||
| 2. 可以掃描記錄各種光學信號（散射、熒光）的動態變化 | |||||
| 3. 可實現高頻、原位分析水體微生物群落及優勢種變化 | |||||
| 4. 可在完整的藻類粒徑譜范圍內對生物量進行線性評估 | |||||
| 5. 可直接分析大尺寸范圍的浮游藻類、團體結構，可現場分析微囊藻群體結構變化 | |||||
| 6. 可調式PMT可根據檢測粒徑大小調節檢測器靈敏度 | |||||
| 7. 流動成像技術可對感興趣感興趣的聚群進行圈門設定后專門拍照 | |||||
| 8. 的脈沖信號指紋圖譜技術，圈門直觀方便，更真實反應細胞形態 | |||||
| 9. 水下測量(CytoSub)可在整個真光層分析浮游植物動態 | |||||
| 10. 可整合入浮標中或其它載體上進行在線監測，可配合CTD對水體做剖面測量 | |||||
| 11.實現實驗室遠程控制基站式自動在線監測，可實現自動檢測，無人值守在線監測 | |||||
| 測量參數 | |||||
| 光學參數： 前向散射FWS、側向散射SWS，熒光散射FLR、 FLY、 FLO | |||||
| 形態參數： 能同時獲得包括細胞和顆粒形態物理特性（數量、長度、大小、形態、粒度、色素、峰數等）、群體特征、脈沖圖譜等在內的9個拓撲學指標及最少45組參數 | |||||
| 計數：自然水體總顆粒計數，圈門后可集群計數及濃度計算，可實現鏈狀藻單細胞數計數功能 | |||||
| 其他測量參數：分析體積、進樣速率等 | |||||
| 應用領域 | |||||
| 1. 海洋生態學與淡水生態學 | |||||
| 2. 流域監測與管理 | |||||
| 3. 海洋學與湖沼學 | |||||
| 4. 有害藻華（HABs）預警 | |||||
| 5. 微藻生物技術 | |||||
| 6. 河流、水庫、湖泊、海洋的監測與管理 | |||||
| 7. 監測與管理 | |||||
| 8. 水源地、水廠、污水處理廠的水質監測 | |||||
| 9. 富營養化研究 | |||||
| 10. 藻類環境生物學 | |||||
| 11. 水產養殖 | |||||
| 選購指南： | |||||
| 一、便攜式浮游植物流式細胞儀CytoSense | |||||
| 系統組成： | |||||
| 流式細胞儀分析主機：相干高質量連續固態激光器，標配波長488nm, 可選波長445nm、635nm、640nm、660nm等，最多可配置7個檢測器（檢測通道含FWS L+R、SWS、YF、RF、OF）。 | |||||
| 野外便攜式外殼：儀器采用碳素纖維外殼，防濺水設計，更輕便（<15kg）,整機安裝于輕質鋁質框，帶高質量防震墊。包裝于便攜式航空箱內。 | |||||
| 數據分析系統：含便攜式筆記本電腦，預裝數據采集軟件CytoUSB,和數據分析軟件CytoClus | |||||
| 批量處理數據分析軟件EasyClus : 需購買MatLab軟件配合使用 | |||||
| 高速流動成像模塊：可選。 | |||||
|  |  | |||
| 便攜式浮游植物流式細胞儀 | Easyclus 粒徑分布圖 | Easyclus 散點圖 | |||
| 系統組成： | |||||
| 主機：淺水版Cytosub （水下20米），含CytoSense所有基本配置 | |||||
| 浮標模塊：包括浮標、太陽能電池板、充電電池、浮標燈、電子系統、無線傳輸裝置和采樣管防水連接器等。根據用戶需要，也可擴展為易拆卸浮標模塊，這樣用戶可以非常方便的在CytoSense（室內用）和CytoBuoy（在線監測）間轉換。 | |||||
| 注意：野外在線監測時不僅于以浮標作為平臺，其他平臺也可，只要可以具備放置CytoSense的空間及供電即可。同時，增加Bacterial staining module，可實現水體異養微生物自動染色和在線分析，可在線檢測藻類、細菌、浮游動物及沉積物等顆粒。具體信息請。 | |||||
| |||||
| CytoBuoy 浮體 | |||||
| CytoBuoy通訊模式：無線通訊 | |||||
| |||||
| 三、水下浮游植物流式細胞儀——CytoSub | |||||
| 主機：臺式機CytoSense是防濺水設計，可以在野外使用，但不能水下使用。CytoSense加上一個水下模塊（SUB MODULE）就組成了水下式流式細胞儀CytoSub。 | |||||
| 水下模塊：一個耐受200 m水深壓力的防水外殼，閥門和進樣環路部分（包括循環泵），電子控制單元，數采，水下連接器和支架。 | |||||
 |  | ||||
| Cytosub 主機 | CytoSense 與CytoSub 轉換 | ||||
| 工作模式一：AUV搭載 | |||||
 | |||||
| 利用英國國家海洋中心AutoSub型AUV搭載CytoSub | |||||
| 工作模式二：水下垂直剖面分析 | |||||
 | |||||
| 與CTD結合一起測量 | |||||
| 注意：此外，水下型浮游植物流式細胞儀CytoSub可應用于浮標，Ferrybox等監測平臺，在垂直剖面不同層位獲取浮游植物生物量信息，對研究微囊藻沉浮機制，浮游動物、水文、水質等因素對浮游植物生態位影響提供數據依據。 | |||||
 |  | ||||
| CytoSense 檢測對象 | |||||
| 產地：荷蘭 CytoBuoy | |||||
| 參考文獻 |
| 數據來源： Cytometry , Goolge scholar等，截至2016年，共收集相關文獻近100篇。 |
1. Simon Bonato a, Elsa Breton , al e: Spatio-temporal patterns in phytoplankton assemblages ininshore–offshore gradients using flow cytometry: A case study in the eastern English Channel, Journal of Marine Systems 2016,76-83.[CytoSense] 2. Goran Bakalar & Vinko Tomas, Possibility of Using Flow Cytometry in the Treated Ballast Water Quality Detection, Pomorski zbornik 51 (2016), 43-55 3. Quan Zhou, Wei Chen, al e: A flow cytometer based protocol for quantitative analysis of bloom-forming cyanobacteria (Microcystis) in lake sediments, Journal of Environmental Sciences 2012, 24(9) 1709–1716 4. A. Mansour, I. Leblond al.e: Invited Paper: Wireless Sensor Networks for Ecosystem Monitoring & Port Surveillance. (WSCN 2013) 5. Endymion D. Cooper , Bastian Bentlage al e: Metatranscriptome profiling of a harmful algal bloom.Harmful Algea 37(2014)75-83. 6. SERGIO A. COELHO-SOUZA, FáBIO V. ARAúJO al e: Bacterial and Archaeal Communities Variability Associated with Upwelling and Anthropogenic Pressures in the Protection Area of Arraial do Cabo (Cabo Frio region - RJ). Anais da Academia Brasileira de Ciências (2015) 87(3):1737-1750 7. Malkassian, A., D. Nerini, al. e: Functional analysis and classification of phytoplankton based on data from an automated flow cytometer. Cytometry Part A 2011, 94A:263-275. [Cytosense] 8. Thyssen, M., B. Beker, al. e: Phytoplankton distribution during two contrasted summers in a Mediterranean harbour: combining automated submersible flow cytometry with conventional techniques. Environmental Monitoring and Assessment 2011, 173:1-16. 9. Thyssen, M., Denis M: Temporal and Spatial High-Frequency Monitoring of Phytoplankton by Automated Flow Cytometry and Pulse-Shape Analysis. Springer Netherlands 2011:293-298. 10. Vidoudez, C., J. C. Nejstgaard, al. e: Dynamics of Dissolved and Particulate Polyunsaturated Aldehydes in Mesocosms Inoculated with Different Densities of the Diatom Skeletonema marinoi. Marine Drugs 2011, 9: 345-358. 11. Hansen, B. W., H. H. Jakobsen, al. e: Swimming behavior and prey retention of the polychaete larvae Polydora ciliata. Journal of Experimental Biology 2010:3237-3246. 12. Pereira GC, Figuiredo ARd, Jabor PM, Ebecken1 NFF: Assessing the ecological status of plankton in Anjos Bay: a flowcytometry approach. Biogeosciences Discuss 2010, 7:6243–6264. [cytobuoy] 13. Barofsky, A., Simonelli P, al e: Growth phase of the diatom Skeletonema marinoi influences the metabolic profile of the cells and the selective feeding of the copepod Calanus spp. J Plankton Res 2009, 32:263-272. [CytoBuoy] 14. Donk V, E., Cerbin S, al e: The effect of a mixotrophic chrysophyte on toxic and colony-forming cyanobacteria. Freshwater Biology 2009, 54:1843-1855. 15. Pereira, C. G, Granato A, al. e: Virioplankton Abundance in Trophic Gradients of an Upwelling Field. Brazilian Journal of Microbiology 2009, 40:857-865. [CytoBuoy] 16. Thyssen, M., Mathieu D, al. e: Short-term variation of phytoplankton assemblages in Mediterranean coastal waters recorded with an automated submerged flow cytometer. J Plankton Res 2008, 30:1027-1040. [Cytosub] 17. Thyssen, T. M, Garcia N, al. e: Sub meso scale phytoplankton distribution in the north east Atlantic surface waters determined with an automated flow cytometer. Biogeosciences Discuss 2008, 5:2471-2503. [Cytosub] 18. Dubelaar, J. GB, Casotti R, al. e: Phytoplankton and their analysis by flow cytometry. Flow Cytometry with Plant Cells 2007:287-322. [CytoBuoy] 19. Takabayashi, M., Lew K, al e: The effect of nutrient availability and temperature on chain length of the diatom, Skeletonema costatum. J Plankton Res 2006, 28:831-840. [CytoSense] 20. Takabayashi, M., Wilkerson FP, al. e: Response Of Glutamine Synthetase Gene Transcription And Enzyme Activity To External Nitrogen Sources In The Diatom Skeletonema Costatum (Bacillariophyceae). J Phycol 2005, 41:84-94. [Cytobuoy] 21. Dubelaar, J. GB, Geerders PJF: Innovative technologies to monitor plankton dynamics. Sea Technol 2004, 45:15-21. [CytoSub] 22. Dubelaar, J. GB, Geerders PJF, al. e: High frequency monitoring reveals phytoplankton dynamics. J Environ Monit 2004, 6:946-952. [Cytosense] 23 Cunninghama, A., McKeea D, al e: Fine-scale variability in phytoplankton community structure and inherent optical properties measured from an autonomous underwater vehicle. J Mar Syst 2003, 43:51-59. 24. Dubelaar, J. GB, Gerritzen PL: CytoBuoy: a step forward towards using flow cytometry in operational oceanography. Sci Mar (Barc) 2000, 64:255-265. [CytoBuoy] 25. Dubelaar, J. GB, Jonker RR: Flow cytometry as a tool for the study of phytoplankton. Scientia Marina 2000, 64. [CytoBuoy] 26. Jonker R, Droben R, Tarran G, Medlin L, Wilkins M, Garcla L, zabala L, boddy l: Automated identification and characterisation of microbial populations using flow cytometry: the AIMS project. scientia marina 2000, 64:225-234. [Cyto] 27. Woodd-Walker, S. R, Gallienne CP, al e: A test model for optical plankton counter (OPC) coincidence and a comparison of OPC-derived and conventional measures of plankton abundance. J Plankton Res 2000, 22:473-483. 28. Dubelaar, J. GB, Gerritzen PL, al e: Design and first results of CytoBuoy: A wireless flow cytometer for in situ analysis of marine and fresh waters. Cytometry 1999, 37:247-254. [CytoBuoy] 29. Wilkins, F. M, Boddy L, al e: Identification of Phytoplankton from Flow Cytometry Data by Using Radial Basis Function Neural Networks." Appl Environ Microbiol 1999, 65:4404-4410. 30. Jonker, R. R, Meulemans JT, al e: Flow cytometry: A powerful tool in analysis of biomass distributions in phytoplankton. Water SciTechnol 1995, 32:177-182. [Cytosense] 31. Jonker, R. R, G. B. J. Dubelaar, al. e: The European Optical Plankton Analyser: A high dynamic range flow cytometer. Scientia Marina 1994. 32. Dubelaar, G. B. J., A. Groenewegen ea: Optical plankton analyser: a flow cytometer for plankton analysis, II: Specifications. Cytometry 1989, 10:529-539. [OPA] 33. Peeters, J. C. H., G. B. J. Dubelaar, al e: Optical plankton analyser: A flow cytometer for plankton analysis, I: Design considerations. Cytometry 1989, 10:522-528. [OPA] |
